細(xì)胞名稱

人臍靜脈內(nèi)皮細(xì)胞 HUVEC

貨物編碼

BFN607200285

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研用途                1類

培養(yǎng)體系

The base medium for this cell line is F-12K Medium (ATCC 30-2004). To make the complete growth medium, add the following components to 440 mL of the base medium:

5 mL of a 10 mg/mL stock heparin solution (prepared from Sigma catalog #H3393) for a final concentration of 0.1 mg/mL heparin in complete growth medium

Dissolve 1 g Heparin in 100 mL basal F-12K and filter to make a 10 mg/mL stock solution

5 mL endothelial cell growth supplement (ECGS; BD Biosciences catalog # 354006)

Rehydrate 1 vial using 5 mL basal F-12K

50 mL fetal bovine serum (FBS; ATCC 30-2020)

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人臍靜脈內(nèi)皮細(xì)胞HUVEC 源于ATCC（CRL-1730）.部分文獻(xiàn)稱該細(xì)胞為HUVEC-C。

注釋

Characteristics: Life expectancy of 50 to 60 population doublings (ATCC). 54 PDL (PubMed=28755030).

Characteristics: Contains an integrated copy of HHV-6B (CI-HHV-6B) at the distal end of chromosome 9 (PubMed=28755030).

Doubling time: ~23.5 hours (between passages 18 and 30), ~67 hours (between passages 24 and 27), ~84 hours (between passages 27 and 30), ~100 hours (between passages 32 and 34) (PubMed=28755030).

STR信息

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參考文獻(xiàn)

PubMed=6333682; DOI=10.1073/pnas.81.20.6413

Hoshi H., McKeehan W.L.

Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture.

Proc. Natl. Acad. Sci. U.S.A. 81:6413-6417(1984)

PubMed=28755030; DOI=10.1007/s10616-017-0119-y

Shioda S., Kasai F., Ozawa M., Hirayama N., Satoh M., Kameoka Y., Watanabe K., Shimizu N., Tang H., Mori Y., Kohara A.

The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture.

Cytotechnology 70:141-152(2018)

PubMed=29892436; DOI=10.1098/rsos.172472

Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M., Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S., Satoh M., Shimizu N., Kohara A.

Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.

R. Soc. Open Sci. 5:172472-172472(2018)