細(xì)胞名稱

人膀胱移形癌細(xì)胞RT112

貨物編碼

BFN60808321

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級

1

用途限制

僅供科研        3類

培養(yǎng)體系

90%DMEM+10%FBS+1%雙抗 

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡介

人膀胱移形癌細(xì)胞RT112取自女性供體，該細(xì)胞源于DSMZ

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: TCGA-110-CL cell line panel.

Part of: UBC-40 urothelial bladder cancer cell line index.

Doubling time: 24 hours (PubMed=3708594); 25 hours (PubMed=25984343); 25.7 hours (PubMed=26055179); ~35 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突變

FGFR3-TACC3 gene fusion (PubMed=25485619; PubMed=28855393).

TERT c.228C>T (-124C>T); in promoter (PubMed=24035680; PubMed=31068700).

Heterozygous for TP53 p.Ser183Ter (c.548C>G) and p.Arg248Gln (c.743G>A) (CCLE; Cosmic-CLP).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        10,11 (CLS; Cosmic-CLP; DSMZ; PubMed=27270441)

10 (PubMed=25877200)

D3S1358        15

D5S818        10,13

D7S820        11,12

D8S1179        13,15

D13S317        13,14

D16S539        11,13

D18S51        15 (CLS)

15,17 (PubMed=25877200)

D21S11        27,30

FGA        23 (CLS)

23,25 (PubMed=25877200)

Penta D        10,11 (CLS)

10 (PubMed=25877200)

Penta E        12,16

TH01        7

TPOX        8,11

vWA        14,17

參考文獻(xiàn)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2

Yu K., Chen B., Aran D., Charalel J., Yau C., Wolf D.M., van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.

Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.

Nat. Commun. 10:3574-3574(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)