細(xì)胞名稱

人非小細(xì)胞肺癌細(xì)胞NCI-H358                  

貨物編碼

BFN60800666

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研用途                  1類

培養(yǎng)體系

DMEM高糖培養(yǎng)基（Hyclone）+10%胎牛血清（Gibco）+1%雙抗（Hyclone）

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人非小細(xì)胞肺癌細(xì)胞NCI-H358細(xì)胞于1981年從一位開始化療之前的患者的腫瘤組織中分離建株。超微結(jié)構(gòu)研究表明細(xì)胞質(zhì)中有Clara細(xì)胞的特征結(jié)構(gòu)細(xì)胞表達(dá)主要的肺表面結(jié)合蛋白SP-A的蛋白和RNA。不表達(dá)SP-B和SP-C。他們?cè)谲洯傊械目寺⌒纬尚?/strong>為0.83%。 

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Part of: NCI RAS program mutant KRAS cell line panel.

Doubling time: 38 hours (in RPMI 1640 + 10% FBS), 76 hours (in ACL-3), 60 hours (in ACL-3 + BSA) (PubMed=3940644); 38 hours (ECACC).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Caution: We are not certain that NCI-H358 and NCI-H358M are identical.

Misspelling: H1358; In PubMed=1311061 table 1.

STR信息

 Amelogenin:X,Y；CSF1PO:11，12；D13S317:8，12；D16S539:12，13；D18S51:14；D19S433:13，14；D21S11:28，30；D2S1338:17，23；D3S1358:14，18；D5S818:10,12；D7S820:10，11；D8S1179:13，14；FGA:20,21；TH01:6；TPOX:8，9；vWA:17

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